How to normalize metatranscriptomic count data for differential expression analysis

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How to normalize metatranscriptomic count data for differential expression analysis

BACKGROUND Differential expression analysis on the basis of RNA-Seq count data has become a standard tool in transcriptomics. Several studies have shown that prior normalization of the data is crucial for a reliable detection of transcriptional differences. Until now it has not been clear whether and how the transcriptomic approach can be used for differential expression analysis in metatranscr...

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Title Tcc: Differential Expression Analysis for Tag Count Data with Robust Normalization Strategies

December 22, 2016 Type Package Title TCC: Differential expression analysis for tag count data with robust normalization strategies Version 1.14.0 Author Jianqiang Sun, Tomoaki Nishiyama, Kentaro Shimizu, and Koji Kadota Maintainer Jianqiang Sun , Tomoaki Nishiyama Description This package provides a series of functions for performing ...

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Package 'tcc' Title Tcc: Differential Expression Analysis for Tag Count Data with Robust Normalization Strategies

April 26, 2017 Type Package Title TCC: Differential expression analysis for tag count data with robust normalization strategies Version 1.16.0 Author Jianqiang Sun, Tomoaki Nishiyama, Kentaro Shimizu, and Koji Kadota Maintainer Jianqiang Sun , Tomoaki Nishiyama Description This package provides a series of functions for performing dif...

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TCC: Differential expression analysis for tag count data with robust normalization strategies

The R/Bioconductor package, TCC, provides users with a robust and accurate framework to perform differential expression (DE) analysis of tag count data. We recently developed a multi-step normalization method (TbT; Kadota et al., 2012 [3]) for two-group RNA-seq data. The strategy (called DEGES) is to remove data that are potential differentially expressed genes (DEGs) before performing the data...

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ژورنال

عنوان ژورنال: PeerJ

سال: 2017

ISSN: 2167-8359

DOI: 10.7717/peerj.3859